• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ATR Polyklonaler Antikörper

ATR Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19787-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using ATR Polyclonal antibody (19787-1-AP)

    WB analysis using 19787-1-AP (same clone as 19787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19787-1-AP (ATR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19787-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATR Polyclonal antibody (19787-1-AP)

    WB analysis of HeLa using 19787-1-AP (same clone as 19787-1-PBS)

    WB result of ATR antibody (19787-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATR transfected HeLa cells. This data was developed using the same antibody clone with 19787-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using ATR Polyclonal antibody (19787-1-AP)

    IP experiment of mouse testis using 19787-1-AP (same clone as 19787-1-PBS)

    IP result of anti-ATR (IP:19787-1-AP, 4ug; Detection:19787-1-AP 1:500) with mouse testis tissue lysate 2040 ug. This data was developed using the same antibody clone with 19787-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

19787-1-PBS bindet in WB, IP, Indirect ELISA ATR und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name ataxia telangiectasia and Rad3 related
Berechnetes Molekulargewicht 301 kDa
Beobachtetes Molekulargewicht 250-290 kDa
GenBank-ZugangsnummerNM_001184
Gene symbol ATR
Gene ID (NCBI) 545
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATR, also named as FRP1, belongs to the PI3/PI4-kinase family and ATM subfamily. ATR is a serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR recognizes the substrate consensus sequence [ST]-Q. ATR phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. It is required for FANCD2 ubiquitination. It is critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. ATR catalyze the reaction: ATP + a protein = ADP + a phosphoprotein. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) which is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. The antibody can recognize all the isoforms of ATR.