ATRX Polyklonaler Antikörper

ATRX Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20495-1-PBS

Synonyme

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  WB analysis using 20495-1-AP (same clone as 20495-1-PBS)

  A549 cells were subjected to Tris-Acetate gel system followed by western blot with 20495-1-AP (ATRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20495-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ATRX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATRX fusion protein Ag13984
• Vollständiger Name: alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
• Berechnetes Molekulargewicht: 2492 aa, 283 kDa
• Beobachtetes Molekulargewicht: 280-300 kDa
• GenBank-Zugangsnummer: BC002521
• Gene symbol: ATRX
• Gene ID (NCBI): 546
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The α-thalassemia mental retardation X-linked protein (ATRX) is a member of the Switch 2, sucrose non-fermenting 2 (SWI2/SNF2) family of helicases/ATPases that exhibits chromatin remodeling activity. ATRX contains an atypical plant homeodomain (PHD) finger domain that recognizes a combinatorial modification pattern on histone H3 tails. ATRX mutations in cancer are most commonly found in pediatric and adolescent malignancies including glioma, neuroblastoma, adrenocortical carcinoma and osteosarcoma(PMID: 32015155).