ATX3,ATXN3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATX3,ATXN3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 13505-1-AP

Synonyme

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

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Galerie der Validierungsdaten

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  WB analysis using 13505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13505-1-AP

  HEK-293 (WT and ATXN3 KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 13505-1-AP incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of mouse heart using 13505-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 13505-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13505-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13505-1-AP

  HEK-293 lysates prepared and IP of ATXN3 performed using 1.0 μg of 13505-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human pancreas cancer using 13505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, HEK-293-Zellen, Mausherzgewebe, Maushirngewebe, Maus-Plazenta-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

13505-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ATX3,ATXN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Caenorhabditis elegans, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATX3,ATXN3 fusion protein Ag4341
• Vollständiger Name: ataxin 3
• Berechnetes Molekulargewicht: 370 aa, 43 kDa
• Beobachtetes Molekulargewicht: 35-42 kDa
• GenBank-Zugangsnummer: BC033711
• Gene symbol: ATXN3
• Gene ID (NCBI): 4287
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

Publikationen

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu ; KD Validated
human	WB, IF, IP	PLoS Genet; The Role of the Mammalian DNA End-processing Enzyme Polynucleotide Kinase 3'-Phosphatase in Spinocerebellar Ataxia Type 3 Pathogenesis.; Authors - Arpita Chatterjee ; KD Validated
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
human	WB	PLoS Genet; Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3.; Authors - Rui Gao
mouse	WB	Neurobiol Dis; A new humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of neurons and glia and late disease onset of SCA3/MJD.; Authors - Pawel M Switonski