ATXN2L Rekombinanter Antikörper

ATXN2L Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251578A9

Kat-Nr. : 86613-3-RR

Synonyme

A2D, A2LG, A2LP, A2RP, ataxin 2 like

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  WB analysis using 86613-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86613-3-RR (ATXN2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 86613-3-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ATXN2L antibody (86613-3-RR, Clone: 251578A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86613-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86613-3-RR against Human ATXN2L were performed. The affinity constant is 1.78 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293T-Zellen, Jurkat-Zellen, MKN-45-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86613-3-RR bindet in WB, IF/ICC, ELISA ATXN2L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATXN2L fusion protein Ag20581
• Vollständiger Name: ataxin 2-like
• Berechnetes Molekulargewicht: 1062 aa, 112 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC082760
• Gene symbol: ATXN2L
• Gene ID (NCBI): 11273
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.