Aurora A Polyklonaler Antikörper

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• KD/KO Validated

Aurora A Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 10297-1-AP

Synonyme

AIK, ARK 1, ARK1, AURA, AURKA, Aurora A, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

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Galerie der Validierungsdaten

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10297-1-AP (Aurora A antibody) at dilution of 1:10 (under 25x lens).

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  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

10297-1-AP bindet in IHC, IF, ELISA Aurora A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Aurora A fusion protein Ag0362
• Vollständiger Name: aurora kinase A
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC002499
• Gene symbol: AURKA
• Gene ID (NCBI): 6790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Publikationen

Species	Application	Title
human		Acta Pharm Sin B; Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway.; Authors - Xiangjin Zheng
mouse	IF	Nat Commun; CPEB1 directs muscle stem cell activation by reprogramming the translational landscape.; Authors - Wenshu Zeng
human		Oncotarget; Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.; Authors - Yanwei Liu
human		Eur J Med Chem; Design, synthesis, biological evaluation of 6-(2-amino-1H-benzo[d]imidazole-6-yl)quinazolin-4(3H)-one derivatives as novel anticancer agents with Aurora kinase inhibition.; Authors - Chengcheng Fan
human		Front Immunol; The ALDH Family Contributes to Immunocyte Infiltration, Proliferation and Epithelial-Mesenchymal Transformation in Glioma.; Authors - Zeyu Wang
human		J Exp Clin Cancer Res; 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.; Authors - Wan Li