AURKA Polyklonaler Antikörper

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• KD/KO Validated

AURKA Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 28371-1-AP

Synonyme

Aurora A, aurora kinase A, AURK, AURA, ARK-1

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  WB analysis using 28371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28371-1-AP (AURKA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 28371-1-AP

  WB result of AURKA antibody (28371-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AURKA transfected HEK-293 cells.

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  IF Staining of HeLa using 28371-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (28371-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Caco-2 using 28371-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using AURKA antibody (28371-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, SW 1990-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

28371-1-AP bindet in WB, IHC, IF/ICC, ELISA AURKA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AURKA fusion protein Ag29006
• Vollständiger Name: aurora kinase A
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC002499
• Gene symbol: AURKA
• Gene ID (NCBI): 6790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Publikationen

Species	Application	Title
human	IHC,IF	Gene; Analysis of ferroptosis-related key genes and regulatory networks in diabetic foot ulcers; Authors - Jianxiong Qiao