AURKA Monoklonaler Antikörper

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AURKA Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F4B10

Kat-Nr. : 66757-1-PBS

Synonyme

Aurora A, 1F4B10, AIRK1, ARK-1, AURA

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  WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 66757-1-Ig (same clone as 66757-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 66757-1-Ig (same clone as 66757-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66757-1-Ig (same clone as 66757-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (66757-1-Ig, Clone: 1F4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50265-1

  Cytometric bead array standard curve of MP50265-1, AURKA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66757-2-PBS. Detection antibody: 66757-1-PBS. Standard:Ag20096. Range: 0.391-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66757-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA AURKA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AURKA fusion protein Ag20096
• Vollständiger Name: aurora kinase A
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46-52 kDa
• GenBank-Zugangsnummer: BC002499
• Gene symbol: AURKA
• Gene ID (NCBI): 6790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).