AURKA Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

AURKA Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230087F7

Kat-Nr. : 82906-2-RR

Synonyme

Aurora A, AURK, AURA, ARK-1, AIRK1

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  WB analysis using 82906-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82906-2-RR (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82906-2-RR

  WB result of AURKA antibody (82906-2-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AURKA transfected HEK-293 cells.

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  IF Staining of HepG2 using 82906-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AURKA antibody (82906-2-RR, Clone: 230087F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  Affinity and Kinetic Characterization of 82906-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82906-2-RR against Human AURKA were performed. The affinity constant is 76.9 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, NCCIT-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82906-2-RR bindet in WB, IF/ICC, ELISA AURKA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AURKA fusion protein Ag0362
• Vollständiger Name: aurora kinase A
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46-52 kDa
• GenBank-Zugangsnummer: BC002499
• Gene symbol: AURKA
• Gene ID (NCBI): 6790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).