Aurora B Monoklonaler Antikörper

Aurora B Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

3H1

Kat-Nr. : 60098-1-Ig

Synonyme

AIK2, AIM 1, AIM1, ARK 2, ARK2, AurB, AURKB, Aurora B, aurora kinase B, Aurora related kinase 2, Aurora/IPL1 related kinase 2, IPL1, STK 1, STK12, STK5

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 60098-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60098-1-Ig (Aurora B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 60098-1-Ig

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 60098-1-Ig (Aurora B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 60098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60098-1-Ig (Aurora B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 60098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60098-1-Ig (Aurora B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

60098-1-Ig bindet in WB, IHC, ELISA Aurora B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aurora B fusion protein Ag7532
• Vollständiger Name: aurora kinase B
• Berechnetes Molekulargewicht: 39.2 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC013300
• Gene symbol: AURKB
• Gene ID (NCBI): 9212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aurora B(AURKB) is also named as aurora kinase B, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5 and belongs to the Aurora subfamily. Aurora kinase B is a chromosomal passenger serine/threonine protein kinase that regulates accurate chromosomal segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachments, and regulation of the mitotic checkpoint(PMID:18311747). Expression levels of AURKB are increased in the anaplastic thyroid carcinoma cell lines ARO, Cal 62, and KAT-4(PMID:15562011).

Publikationen

Species	Application	Title
human	WB	Gastroenterology; Overexpression of Rac GTPase Activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis.; Authors - Xiao-Mei Yang
mouse	WB	Hum Mol Genet; Downregulation of Survivin contributes to cell-cycle arrest during postnatal cardiac development in a severe spinal muscular atrophy mouse model.; Authors - Lei Sheng
human	WB	PLoS One; Anti-pancreatic cancer deliverables from sea: first-hand evidence on the efficacy, molecular targets and mode of action for multifarious polyphenols from five different brown-algae.; Authors - Aravindan Sheeja S
human	WB	FEBS J; YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells; Authors - Shu-Er Chow