AUTS2 Polyklonaler Antikörper

AUTS2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 25001-1-AP

Synonyme

AUTS2, FBRSL2, KIAA0442

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Galerie der Validierungsdaten

• 

  WB analysis of C6 using 25001-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25001-1-AP (AUTS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25001-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25001-1-AP (AUTS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

25001-1-AP bindet in WB, IF, ELISA AUTS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AUTS2 fusion protein Ag21853
• Vollständiger Name: autism susceptibility candidate 2
• Berechnetes Molekulargewicht: 139 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 130-140 kDa
• GenBank-Zugangsnummer: BC064693
• Gene symbol: AUTS2
• Gene ID (NCBI): 26053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AUTS2 also named as FBRSL2 or autism susceptibility candidate 2 is a 1259 amino acid protein, which belongs to the AUTS2 family. AUTS2 is highly expressed in brain, kidney and skeletal muscle. AUTS2 exists as four isoforms produced by alternative splicing events and is encoded by a gene located on human chromosome 7. The molecular weight of AUTS2 isoform is 65-70 kDa in the brain ( PMID:23332918). The functional role of AUTS2 is not well known, although some studies have identified a putative role in transcriptional regulation during neuronal development.

Publikationen

Species	Application	Title
human	IF	Front Neurol; Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.; Authors - Jing Huang
human	IF	Neuroscience; PMID: 30599272; Authors - Fangkun Liu
mouse		Mol Cell; Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities.; Authors - Andrea Scelfo
mouse	WB	Exp Neurol; Auts2 regulated autism-like behavior, glucose metabolism and oxidative stress in mice; Authors - Min Liu