AXIN2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AXIN2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

39

Rezensionen (2)

Kat-Nr. : 20540-1-AP

Synonyme

AXIL, axin 2, Axin like protein, AXIN2, Axis inhibition protein 2, Conductin

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Galerie der Validierungsdaten

• 

  WB analysis using 20540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20540-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 20540-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 20540-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 20540-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20540-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, BxPC-3-Zellen, HeLa-Zellen, Jurkat-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 38 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

20540-1-AP bindet in WB, IHC, IF, ELISA AXIN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AXIN2 fusion protein Ag14574
• Vollständiger Name: axin 2
• Berechnetes Molekulargewicht: 843 aa, 94 kDa
• Beobachtetes Molekulargewicht: 94-100 kDa
• GenBank-Zugangsnummer: BC006295
• Gene symbol: AXIN2
• Gene ID (NCBI): 8313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutaion is involved in colorectal cancer. Catalgo#20540-1-AP is a rabbit polyclonal antibdy raised against a 34 amino acid N-terminal of human AXIN2. Observed MW of AXIN2 is from 93-100 kDa (PMID: 11809809; 24607787).

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Adv Healthc Mater; Ultrathin 2D Titanium Carbide MXene (Ti3 C2 Tx ) Nanoflakes Activate WNT/HIF-1α-Mediated Metabolism Reprogramming for Periodontal Regeneration.; Authors - Di Cui
human	WB	Autophagy; TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.; Authors - Cefan Zhou
human	WB	Theranostics; Targeted inhibition of KDM6 histone demethylases eradicates tumor-initiating cells via enhancer reprogramming in colorectal cancer.; Authors - Junbao Zhang
human	WB	Oncogene; Identification of a novel Calpain-2-SRC feed-back loop as necessity for β-Catenin accumulation and signaling activation in hepatocellular carcinoma.; Authors - Xiao-Lu Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aurélie (Verified Customer) (10-06-2023)

This antibody gives a specific band at 100 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: RKO

AXIN2 Antibody Western Blot validation (1/2000 dilution) in RKO (Cat no:20540-1-AP)

FH

Guannan (Verified Customer) (01-31-2019)

This antibody gives me a band at 95 kd, but also a lot of nonspecific bands.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7

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