AXIN2 Monoklonaler Antikörper

AXIN2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11A4

Kat-Nr. : 67458-1-Ig

Synonyme

Axin-2, Axil, axin 2, Axin like protein, Axin-like protein

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  WB analysis using 67458-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67458-1-Ig (AXIN2 antibody) at dilution of 1:4800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, HepG2-Zellen, Jurkat-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:9600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67458-1-Ig bindet in WB, ELISA AXIN2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AXIN2 fusion protein Ag29291
• Vollständiger Name: axin 2
• Berechnetes Molekulargewicht: 843 aa, 94 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC006295
• Gene symbol: AXIN2
• Gene ID (NCBI): 8313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutation is involved in colorectal cancer.