AXL Polyklonaler Antikörper

AXL Polyklonal Antikörper für FC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 13196-1-AP

Synonyme

AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO

---

Galerie der Validierungsdaten

• 

  WB analysis using 13196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13196-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13196-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 13196-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13196-1-AP

  IP result of anti-AXL(IP:13196-1-AP, 4ug; Detection:13196-1-AP 1:3000) with HeLa cells lysate 1320 ug.

• 

  FC experiment of HeLa using 13196-1-AP

  1X10^6 HeLa cells were stained with 0.2ug AXL antibody (13196-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, HepG2-Zellen, HT-1080.Zellen, humanes Lebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

13196-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA AXL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AXL fusion protein Ag3898
• Vollständiger Name: AXL receptor tyrosine kinase
• Berechnetes Molekulargewicht: 894 aa, 98 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC032229
• Gene symbol: AXL
• Gene ID (NCBI): 558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

Publikationen

Species	Application	Title
human	WB	Int J Cancer; Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.; Authors - Yingying Shen
rat	WB	Int J Mol Sci; Embryonic-Derived Myb- Macrophages Enhance Bacterial Clearance and Improve Survival in Rat Sepsis.; Authors - Mirjana Jerkic
human	WB	Int J Mol Sci; Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.; Authors - Elisa De Tomi
	FC	Bioorg Chem; Non-volatile acylphloroglucinol components from Eucalyptus robusta inhibit Zika virus by impairing RdRp activity of NS5.; Authors - Zhai-Wen Yao
human	WB, IHC,IF	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
human	WB	iScience; SUFU suppresses ferroptosis sensitivity in breast cancer cells via Hippo/YAP pathway.; Authors - Kun Fang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Marta (Verified Customer) (05-30-2022)

The antibody works well. Non-specific bands are not detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A375 melanoma cell line

Verwandte Produkte

GAS6 Polyclonal antibody

13795-1-AP

PI3 Kinase p85 Alpha Monoclonal antibody

60225-1-Ig

PI3 Kinase p55 Gamma Polyclonal antibody

55036-1-AP

GRB2 Polyclonal antibody

10254-2-AP

ACK1 Polyclonal antibody

14304-1-AP