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AXL Monoklonaler Antikörper

AXL Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D12F11

Kat-Nr. : 68108-1-PBS

Synonyme

3D12F11, EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO

Filter:
  • Western Blot (WB) analysis of various lysates using AXL Monoclonal antibody (68108-1-Ig)

    WB analysis using 68108-1-Ig (same clone as 68108-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68108-1-Ig (AXL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68108-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AXL Monoclonal antibody (68108-1-Ig)

    WB analysis of HeLa using 68108-1-Ig (same clone as 68108-1-PBS)

    WB result of AXL antibody (68108-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AXL transfected HeLa cells. This data was developed using the same antibody clone with 68108-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68108-1-PBS bindet in WB, Indirect ELISA AXL und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen AXL fusion protein Ag14401
Vollständiger Name AXL receptor tyrosine kinase
Berechnetes Molekulargewicht 894 aa, 98 kDa
Beobachtetes Molekulargewicht130-140 kDa
GenBank-ZugangsnummerBC032229
Gene symbol AXL
Gene ID (NCBI) 558
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).