Acetyl-Histone H2B (Lys120) Rekombinanter Antikörper

Acetyl-Histone H2B (Lys120) Rekombinant Antikörper für WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

241556E7

Kat-Nr. : 84551-1-PBS

Synonyme

Acetyl Histone, H2B Ac Lys120, H2B Acetyl K120, H2B Acetyl Lys120, H2B Acetyl Lysine120

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  WB analysis of NIH/3T3 using 84551-1-RR (same clone as 84551-1-PBS)

  Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84551-1-RR (same clone as 84551-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 84551-1-RR (same clone as 84551-1-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 84551-1-RR (same clone as 84551-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys120) (84551-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84551-1-PBS bindet in WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR Acetyl-Histone H2B (Lys120) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 2, H2be
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC005827
• Gene symbol: Histone H2B
• Gene ID (NCBI): 8349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).