Acetyl-Histone H2B (Lys15) Rekombinanter Antikörper

Acetyl-Histone H2B (Lys15) Rekombinant Antikörper für WB, Dot Blot, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Dot Blot, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

250366G3

Kat-Nr. : 83171-7-RR

Synonyme

Ac Histone H2B, Ac Histone H2B (Lys15), Ac Histone H2B Lys15, Ac Histone H2B-Lys15, Acetyl Histone

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  WB analysis of NIH/3T3 using 83171-7-RR

  Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-7-RR (Acetyl-Histone H2B (Lys15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

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  Dot Blot experiment using 83171-7-RR

  Dot blot analysis was used to confirm the specificity of 83171-7-RR Acetyl-Histone H2B (Lys15) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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  ChIP experiment of HeLa using 83171-7-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys15) (83171-7-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Trichostatin A treated NIH/3T3 cells
• Erfolgreiche Detektion in Dot Blot: N/A
• Erfolgreiche Detektion in ChIP-qPCR: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• DOT BLOT: DOT BLOT : 1:500-1:2000
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83171-7-RR bindet in WB, Dot Blot, ELISA, ChIP-qPCR Acetyl-Histone H2B (Lys15) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 2, H2be
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC005827
• Gene symbol: Histone H2B
• Gene ID (NCBI): 8349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.