Acetyl-Histone H2B (Lys16) Rekombinanter Antikörper

Acetyl-Histone H2B (Lys16) Rekombinant Antikörper für WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

241194H2

Kat-Nr. : 84446-1-RR

Synonyme

Acetyl Histone, H2B Ac Lys16, H2B Acetyl K16, H2B Acetyl Lys16, H2B Acetyl Lysine16

---

• 

  WB analysis of NIH/3T3 using 84446-1-RR

  Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

• 

  WB analysis of HSC-T6 using 84446-1-RR

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 84446-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 84446-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H2B antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:850 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 84446-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys16) antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of NIH/3T3 using 84446-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H2B (Lys16) antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Dot Blot experiment of peptide using 84446-1-RR

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys16) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

• 

  ChIP experiment of HeLa using 84446-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys16) (84446-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Trichostatin A treated NIH/3T3 cells, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: Trichostatin A treated HeLa cells, HeLa-Zellen
• Erfolgreiche Detektion in Dot Blot: peptide
• Erfolgreiche Detektion in ChIP-qPCR: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84446-1-RR bindet in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR Acetyl-Histone H2B (Lys16) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 2, H2be
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC005827
• Gene symbol: Histone H2B
• Gene ID (NCBI): 8349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.