Acetyl-Histone H3 (Lys23) Rekombinanter Antikörper

Acetyl-Histone H3 (Lys23) Rekombinant Antikörper für IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

241137E4

Kat-Nr. : 82838-2-PBS

Synonyme

Acetyl Histone, H3 Ac Lys23, H3 Acetyl K23, H3 Acetyl Lys23, H3 Acetyl Lysine23

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  IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82838-2-RR (same clone as 82838-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 82838-2-RR (same clone as 82838-2-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 82838-2-RR (same clone as 82838-2-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys23) (82838-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82838-2-PBS bindet in IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR Acetyl-Histone H3 (Lys23) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.