Acetyl-Histone H3 (Lys27) Rekombinanter Antikörper

Acetyl-Histone H3 (Lys27) Rekombinant Antikörper für WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

1M16

Kat-Nr. : 82902-1-PBS

Synonyme

Ac Histone H3, Ac Histone H3 (Lys27), Ac Histone H3 Lys27, Ac Histone H3-Lys27, Acetyl Histone

---

• 

  WB analysis of HeLa using 82902-1-RR (same clone as 82902-1-PBS)

  Various cell lysates were subjected to SDS PAGE followed by western blot with 82902-1-RR Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 82902-1-RR (same clone as 82902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82902-1-RR (Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82902-1-RR (same clone as 82902-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys27) antibody (82902-1-RR, Clone: 1M16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of peptide using 82902-1-RR (same clone as 82902-1-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys27) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ug/mL) spotted is indicated next to each row. Column 1: H3K27ac. Column 2: Unmodified H3K27. Column 3: H3K9ac. Column 4: H3K14ac. Column 5: H3K18ac. Column 6: H3K23ac. Column 7: H3K36ac. Column 8: H4K5ac. Column 9: H4K8ac. Column 10: H4K12ac. Column 11: H4K16ac. Column 12: H2AK5ac. Column 13: Blank(PBS). This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 82902-1-RR (same clone as 82902-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys27) (82902-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

82902-1-PBS bindet in WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR Acetyl-Histone H3 (Lys27) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Acetyl-Histone H3 (Lys27) is enhancer specific mark and plays positive role in gene expression.