Acetyl-Histone H3 (Lys36) Rekombinanter Antikörper

Acetyl-Histone H3 (Lys36) Rekombinant Antikörper für WB, Dot Blot, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Dot Blot, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

250370A9

Kat-Nr. : 82828-14-RR

Synonyme

Ac Histone H3, Ac Histone H3 (Lys36), Ac Histone H3 Lys36, Ac Histone H3-Lys36, Acetyl Histone

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  WB analysis using 82828-14-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82828-14-RR (Acetyl-Histone H3 (Lys36) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Dot Blot experiment using 82828-14-RR

  Dot blot analysis was used to confirm the specificity of 82828-14-RR Acetyl-Histone H3(Lys36) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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  ChIP experiment of HeLa using 82828-14-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of HIST1H3A (82828-14-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Trichostatin A treated NIH/3T3 cells
• Erfolgreiche Detektion in Dot Blot: N/A N/A
• Erfolgreiche Detektion in ChIP-qPCR: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82828-14-RR bindet in WB, Dot Blot, ELISA, ChIP-qPCR Acetyl-Histone H3 (Lys36) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

H3K36ac peaks at the 5' end of genes,manily on the two nucleosomeus immediately distal to the transcription start site, independently of gene length. H3K36ac and H3K36mac showed negative creastalk, which is mediated by GCN3 and the histone methyl transferase SDX8. Although H3K36ac is associated with gene activity, there is no linear relationship between H3K36ac and transcript levels, suggesting that H3K36ac is a binary indicator of transcription.