Acetyl-Histone H3 (Lys9) Rekombinanter Antikörper

Acetyl-Histone H3 (Lys9) Rekombinant Antikörper für WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

241596F9

Kat-Nr. : 84543-1-PBS

Synonyme

Acetyl Histone, H3 Ac Lys9, H3 Acetyl K9, H3 Acetyl Lys9, H3 Acetyl Lysine9

---

• 

  WB analysis of HEK-293 using 84543-1-RR (same clone as 84543-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of peptide using 84543-1-RR (same clone as 84543-1-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys9) (84543-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys9) (84543-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

84543-1-PBS bindet in WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR Acetyl-Histone H3 (Lys9) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.