ADSL Polyklonaler Antikörper

ADSL Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15264-1-PBS

Synonyme

EC:4.3.2.2, ASL, ASASE, AMPS

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  WB analysis using 15264-1-AP (same clone as 15264-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15264-1-AP (same clone as 15264-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 15264-1-AP (same clone as 15264-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15264-1-AP (same clone as 15264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15264-1-AP (same clone as 15264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15264-1-AP (same clone as 15264-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Adenylosuccinate lyase antibody (15264-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 15264-1-AP (same clone as 15264-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Adenylosuccinate lyase Polyclonal antibody (15264-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15264-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA ADSL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADSL fusion protein Ag7332
• Vollständiger Name: adenylosuccinate lyase
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC000253
• Gene symbol: ADSL
• Gene ID (NCBI): 158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADSL(adenylosuccinate lyase) is also named as AMPS, ASase, ASL and belongs to the lyase 1 family. It is an enzyme involved in 2 pathways of purine nucleotide metabolism and catalyzes cleavage of succinyl groups to yield fumarate(PMID:18524658). Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency). In humans, mutations in ADSL lead to an inborn error of metabolism originally characterized by developmental delay, often with autistic features(PMID:20884265)..The ADSL enzymatic activity is reduced in lymphocytes and red blood cells of the patient with severe psychomotor retardation(PMID:9545543). It has 2 isoforms produced by alternative splicing.