Adiponectin Polyklonaler Antikörper

Adiponectin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30723-1-AP

Synonyme

Adipoq, Acrp30, adipo, apM1

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  WB analysis using 30723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30723-1-AP (Adiponectin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 30723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 30723-1-AP (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 30723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 30723-1-AP (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 30723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Adiponectin antibody (30723-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Fettgewebe, mouse serum
• Erfolgreiche Detektion in IHC: braunes Mausfettgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30723-1-AP bindet in WB, IHC, IF/ICC, ELISA Adiponectin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Adiponectin fusion protein Eg0262
• Vollständiger Name: adiponectin, C1Q and collagen domain containing
• Berechnetes Molekulargewicht: 27kd
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: NM_009605
• Gene symbol: Adiponectin
• Gene ID (NCBI): 11450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.