Adiponectin Rekombinanter Antikörper

Adiponectin Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230258C5

Kat-Nr. : 83070-3-PBS

Synonyme

Adipoq, apM1, adipo, Acrp30, 230258C5

---

• 

  Sandwich ELISA standard curve of MP00039-3

  Sandwich ELISA standard curve of MP00039-3, MOUSE Adiponectin Recombinant Matched Antibody Pair - PBS only. 83070-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0262. 83070-3-PBS was HRP conjugated as the detection antibody. Range:15.6-1000pg/mL.

• 

  Sample test of MP00039-3

  Serum of sixteen mice was measured. The Adiponectin concentration of detected samples was determined to be 7.30 ug/mL with a range of 4.72-13.56 ug/mL.

• 

  Sample test of MP00039-3

  lungs from one mouse were chopped into 1-2 mm pieces and seeded into approximately 30 mL of media containing RPMI, 10% fetal bovine serum, 50 μM β-mercaptoethanol, and L-glutamine.The mean Adiponectin concentration was determined to be 4.4 ng/mL in lung cell culture supernatant.

• 

  Sample test of MP00039-3

  Organs from 2 mice were rinsed with PBS to remove excess blood, chopped into 1-2 mm pieces, homogenized in 5-10 mL of PBS in a tissue homogenizer, and stored at ≤ -80℃ 5 min. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g to remove particulate. The mean Adiponectin concentration was determined to be 130.21 ng/mL in spleen homogenates, 745.9 ng/mL in fat tissue homogenates.

---

Geprüfte Anwendungen

Produktinformation

83070-3-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test Adiponectin und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: adiponectin, C1Q and collagen domain containing
• Berechnetes Molekulargewicht: 27kd
• GenBank-Zugangsnummer: NM_009605
• Gene symbol: Adiponectin
• Gene ID (NCBI): 11450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.