Adiponectin receptor Rekombinanter Antikörper

Adiponectin receptor Rekombinant Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

242777F3

Kat-Nr. : 85121-4-RR

Synonyme

ADIPOR2, ADIPOR1, Adiponectin receptor protein 2, adiponectin receptor 2, ACDCR2

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  WB analysis using 85121-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85121-4-RR (ADIPOR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 85121-4-RR

  IP result of anti-Adiponectin receptor (IP:85121-4-RR, 4ug; Detection:85121-4-RR 1:2000) with mouse liver tissue lysate 2400 ug.

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  IHC staining of mouse liver using 85121-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 85121-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 85121-4-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 85121-4-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 85121-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (85121-4-RR, Clone: 242777F3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 85121-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (85121-4-RR, Clone: 242777F3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85121-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85121-4-RR against Human Adiponectin receptor were performed. The affinity constant is 2.85 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, humanes Plazenta-Gewebe, L02-Zellen, Ratten-Uterusgewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Rattenlebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85121-4-RR bindet in WB, IHC, IF-P, IP, ELISA Adiponectin receptor und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Adiponectin receptor fusion protein Ag5744
• Vollständiger Name: adiponectin receptor 2
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC051858
• Gene symbol: Adiponectin receptor
• Gene ID (NCBI): 79602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.