Adiponectin Rekombinanter Antikörper

Adiponectin Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240392D6

Kat-Nr. : 83525-2-RR

Synonyme

Adipoq, Acrp30, Acdc, 240392D6

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  WB analysis of mouse adipose using 83525-2-RR

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat adipose using 83525-2-RR

  rat adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adiponectin antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 83525-2-RR

  rat heart tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83525-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83525-2-RR against Rat Adiponectin were performed. The affinity constant is 0.149 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: rat adipose tissue, Maus-Fettgewebe, Rattenherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:15000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83525-2-RR bindet in WB, ELISA Adiponectin und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: adiponectin, C1Q and collagen domain containing
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26-28 kDa
• GenBank-Zugangsnummer: NM_144744.3
• Gene symbol: Adiponectin
• Gene ID (NCBI): 246253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.