YTHDF1 Rekombinanter Antikörper

YTHDF1 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241445B10

Kat-Nr. : 80876-2-PBS

Synonyme

241445B10, C20orf21, DACA-1, DF1, YTH domain-containing family protein 1

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  WB analysis using 80876-2-RR (same clone as 80876-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80876-2-RR (YTHDF1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 80876-2-RR (same clone as 80876-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 80876-2-RR (same clone as 80876-2-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80876-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80876-2-RR against Human YTHDF1 were performed. The affinity constant is 7.23 nM.

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Geprüfte Anwendungen

Produktinformation

80876-2-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA YTHDF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: YTHDF1 fusion protein Ag11509
• Vollständiger Name: YTH domain family, member 1
• Berechnetes Molekulargewicht: 559 aa, 61 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC050284
• Gene symbol: YTHDF1
• Gene ID (NCBI): 54915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.