Ajuba Polyklonaler Antikörper

Ajuba Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 28686-1-AP

Synonyme

Ajuba, JUB, Protein ajuba

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  WB analysis using 28686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28686-1-AP (Ajuba antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushautgewebe, humanes Schilddrüsenkarzinomgewebe, Maus-Dünndarmgewebe, Maushodengewebe, Rattenhautgewebe, Ratten-Dünndarmgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

28686-1-AP bindet in WB, IHC, ELISA Ajuba und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ajuba fusion protein Ag29725
• Vollständiger Name: jub, ajuba homolog (Xenopus laevis)
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC007580
• Gene symbol: Ajuba
• Gene ID (NCBI): 84962
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ajuba belongs to the LIM domain proteins, which are involved in the assembly of the extracellular matrix and, along with associated proteins, regulate target genes that connect the extracellular matrix and the cytoskeleton. Ajuba consists of three LIM domains at the C-terminal and is located in the cytoplasm where it interacts with a variety of other proteins. Ajuba contributes to the formation or strengthening of cell-cell adhesion and regulates cell growth and differentiation decisions. Ajuba participates in the assembly of the extracellular matrix. Ajuba also plays an important role in some diseases.

Publikationen

Species	Application	Title
human	WB	Cell Rep; ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression; Authors - Yu Zhang