AlaRS Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

AlaRS Monoklonal Antikörper für WB, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C8H3

Kat-Nr. : 67909-1-Ig

Synonyme

AARS, 1C8H3, AARS1, Alanine--tRNA ligase, cytoplasmic, Alanyl-tRNA synthetase

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  WB analysis using 67909-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AlaRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  WB analysis of HeLa using 67909-1-Ig

  WB result of AlaRS antibody (67909-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HeLa cells.

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  WB analysis using 67909-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse liver using 67909-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 67909-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 67909-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of A431 using 67909-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF-P: Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A431-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67909-1-Ig bindet in WB, IF/ICC, IF-P, ELISA AlaRS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AlaRS fusion protein Ag11151
• Vollständiger Name: alanyl-tRNA synthetase
• Berechnetes Molekulargewicht: 968 aa, 107 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC011451
• Gene symbol: AlaRS
• Gene ID (NCBI): 16
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).