Aldolase C Monoklonaler Antikörper

Aldolase C Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5H7A5

Kat-Nr. : 66120-1-PBS

Synonyme

ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase

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  WB analysis using 66120-1-Ig (same clone as 66120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66120-1-Ig (same clone as 66120-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66120-1-Ig (same clone as 66120-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51427-1

  Cytometric bead array sample test of MP51427-1, ALDOC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66120-1-PBS. Detection antibody: 66120-2-PBS.

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  Cytometric bead array standard curve of MP51427-1

  Cytometric bead array standard curve of MP51427-1, ALDOC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66120-1-PBS. Detection antibody: 66120-2-PBS. Standard:Ag6779. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66120-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Aldolase C und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aldolase C fusion protein Ag6779
• Vollständiger Name: aldolase C, fructose-bisphosphate
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC003613
• Gene symbol: Aldolase C
• Gene ID (NCBI): 230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).