Alix Monoklonaler Antikörper

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Alix Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H9D9

Kat-Nr. : 67715-1-PBS

Synonyme

PDCD6IP, 1H9D9, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X

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  WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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  WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67715-1-Ig (same clone as 67715-1-PBS)

  WB result of Alix antibody (67715-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67715-1-Ig (same clone as 67715-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67715-1-Ig (same clone as 67715-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67715-1-Ig (same clone as 67715-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Alix antibody (67715-1-Ig, Clone: 1H9D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67715-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Alix und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alix fusion protein Ag30437
• Vollständiger Name: programmed cell death 6 interacting protein
• Berechnetes Molekulargewicht: 868 aa, 96 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC020066
• Gene symbol: Alix
• Gene ID (NCBI): 10015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.