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Alix Rekombinanter Antikörper

Alix Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251574C12

Kat-Nr. : 86644-1-PBS

Synonyme

PDCD6IP, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X, DRIP4

Filter:
  • Western Blot (WB) analysis of various lysates using Alix Recombinant monoclonal antibody (86644-1-RR)

    WB analysis using 86644-1-RR (same clone as 86644-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86644-1-RR (ALIX; AIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86644-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86644-1-RR

    Affinity and Kinetic Characterization of 86644-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86644-1-RR against Human Alix were performed. The affinity constant is 6.12 nM.

Geprüfte Anwendungen

Produktinformation

86644-1-PBS bindet in WB, Indirect ELISA Alix und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Alix fusion protein Ag3074
Vollständiger Name programmed cell death 6 interacting protein
Berechnetes Molekulargewicht 868 aa, 96 kDa
Beobachtetes Molekulargewicht100 kDa
GenBank-ZugangsnummerBC020066
Gene symbol Alix
Gene ID (NCBI) 10015
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.