Alix Rekombinanter Antikörper

Alix Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251574C12

Kat-Nr. : 86644-1-RR

Synonyme

PDCD6IP, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X, DRIP4

---

• 

  WB analysis using 86644-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86644-1-RR (ALIX; AIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86644-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86644-1-RR against Human Alix were performed. The affinity constant is 6.12 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, Jurkat-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86644-1-RR bindet in WB, ELISA Alix und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Alix fusion protein Ag3074
• Vollständiger Name: programmed cell death 6 interacting protein
• Berechnetes Molekulargewicht: 868 aa, 96 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC020066
• Gene symbol: Alix
• Gene ID (NCBI): 10015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.