Alpha 1 Antitrypsin Monoklonaler Antikörper

Alpha 1 Antitrypsin Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

1A9G6

Kat-Nr. : 66135-1-Ig

Synonyme

A1A, A-1-Antitrypsin, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT, α1-antitrypsin

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Galerie der Validierungsdaten

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  WB analysis using 66135-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66135-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66135-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66135-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66135-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66135-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66135-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human saliva using 66135-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 66135-1-Ig

  IP Result of anti-Alpha-1-Antitrypsin (IP:66135-1-Ig, 5ug; Detection:66135-1-Ig 1:1000) with human plasma lysate 4000ug.

• 

  IHC staining of human liver using 66135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human liver using 66135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human liver using 66135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66135-1-Ig(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66135-1-Ig(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 66135-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha 1 Antitrypsin antibody (66135-1-Ig, Clone: 1A9G6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 66135-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha 1 Antitrypsin (66135-1-Ig, Clone:1A9G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Hausschwein-Lebergewebe, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

66135-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA Alpha 1 Antitrypsin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha 1 Antitrypsin fusion protein Ag9516
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• Berechnetes Molekulargewicht: 418 aa, 47 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC015642
• Gene symbol: SERPINA1
• Gene ID (NCBI): 5265
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

Publikationen

Species	Application	Title
human	IF	Liver Int; Extensively expanded murine-induced hepatic stem cells maintain high-efficient hepatic differentiation potential for repopulation of injured livers.; Authors - Bing Yu
human	WB	Biochem J; LMAN1-MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin.; Authors - Yuan Zhang
human	IHC	Dis Markers; Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma; Authors - Xiubing Chen
human	WB,IF	Ann Transl Med; Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation.; Authors - Sang Luo
human	WB,IF	Ann Transl Med; Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.; Authors - Sang Luo
human		Blood Adv; Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of FV and FVIII; Authors - Yuan Zhang