Alpha 1B-Glycoprotein Polyklonaler Antikörper

Alpha 1B-Glycoprotein Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14181-1-PBS

Synonyme

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  WB analysis of human plasma using 14181-1-AP (same clone as 14181-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14181-1-AP (same clone as 14181-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 14181-1-AP (same clone as 14181-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 14181-1-AP (same clone as 14181-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 14181-1-AP (same clone as 14181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 14181-1-AP (same clone as 14181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14181-1-AP (same clone as 14181-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:25 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14181-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Alpha 1B-Glycoprotein und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha 1B-Glycoprotein fusion protein Ag5414
• Vollständiger Name: alpha-1-B glycoprotein
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC035719
• Gene symbol: A1BG
• Gene ID (NCBI): 1
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).