Alpha-2-Macroglobulin Monoklonaler Antikörper

Alpha-2-Macroglobulin Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4B11F7

Kat-Nr. : 66126-1-PBS

Synonyme

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M

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  WB analysis of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66126-1-Ig (same clone as 66126-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 66126-1-Ig (same clone as 66126-1-PBS)

  IP result of anti-Alpha-2-Macroglobulin (IP:66126-1-Ig, 5ug; Detection:66126-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (66126-1-Ig, Clone: 4B11F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66126-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Alpha-2-Macroglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha-2-Macroglobulin fusion protein Ag19245
• Vollständiger Name: alpha-2-macroglobulin
• Berechnetes Molekulargewicht: 1474 aa, 163 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC040071
• Gene symbol: Alpha 2 Macroglobulin
• Gene ID (NCBI): 2
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.