Alpha Adaptin Polyklonaler Antikörper

Alpha Adaptin Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11401-1-PBS

Synonyme

AP2A1, Alpha1 adaptin, Alpha adaptin A, ADTAA, Adaptor-related protein complex 2 subunit alpha-1

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  WB analysis of HEK-293 using 11401-1-AP (same clone as 11401-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 11401-1-AP (same clone as 11401-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 11401-1-AP (same clone as 11401-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 11401-1-AP (same clone as 11401-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11401-1-AP (alpha Adaptin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11401-1-AP (same clone as 11401-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Adaptin antibody (11401-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11401-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Alpha Adaptin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha Adaptin fusion protein Ag1927
• Vollständiger Name: adaptor-related protein complex 2, alpha 1 subunit
• Berechnetes Molekulargewicht: 977 aa, 108 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC014214
• Gene symbol: AP2A1
• Gene ID (NCBI): 160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.