Alpha Synuclein Monoklonaler Antikörper

Alpha Synuclein Monoklonal Antikörper für WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B10E9

Kat-Nr. : 66412-1-PBS

Synonyme

1, a-synuclein, SNCA, 1B10E9, Alpha-synuclein

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  WB analysis of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66412-1-Ig (same clone as 66412-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50043-1

  Sandwich ELISA standard curve of MP50043-1, a-Synuclein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60285-1-PBS. Detection antibody: 66412-1-PBS. Standard: Ag1285. Range: 0.313-20 ng/mL.

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  IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66412-1-Ig (a-Synuclein antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody (66412-1-Ig, Clone: 1B10E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody (66412-1-Ig, Clone: 1B10E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66412-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66412-1-Ig against Human SNCA were performed. The affinity constant is 0.388 nM.

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Geprüfte Anwendungen

Produktinformation

66412-1-PBS bindet in WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA Alpha Synuclein und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha Synuclein fusion protein Ag1285
• Vollständiger Name: synuclein, alpha (non A4 component of amyloid precursor)
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 18-19 kDa
• GenBank-Zugangsnummer: BC013293
• Gene symbol: Alpha Synuclein
• Gene ID (NCBI): 6622
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.