Amphiphysin Polyklonaler Antikörper

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• KD/KO Validated

Amphiphysin Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13379-1-PBS

Synonyme

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  WB analysis of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 13379-1-AP (same clone as 13379-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

  IP result of anti-Amphiphysin (IP:13379-1-AP, 4ug; Detection:13379-1-AP 1:1500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13379-1-PBS bindet in WB, IHC, IP, Indirect ELISA Amphiphysin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Amphiphysin fusion protein Ag4204
• Vollständiger Name: amphiphysin
• Berechnetes Molekulargewicht: 695 aa, 76 kDa
• Beobachtetes Molekulargewicht: 115-125 kDa
• GenBank-Zugangsnummer: BC034376
• Gene symbol: AMPH
• Gene ID (NCBI): 273
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Amphiphysin (AMPH), a synaptic vesicle-associated protein that is highly concentrated in nerve terminal, is an autoantigen of Stiff-Man syndrome with breast cancer. Amphiphysin contains a N-terminal BAR (Bin1/Amphiphysin/Rv167) domain followed by an endocytosis domain, and a C-terminal SH3 domain. Amphiphysin has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. It may also participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. (PMID: 8552632; 8245793; 10559861)