Amphiregulin Monoklonaler Antikörper

Amphiregulin Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1G9

Kat-Nr. : 66433-1-PBS

Synonyme

AREG, 1A1G9, AR, AREGB, Colorectum cell-derived growth factor

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  WB analysis of A549 using 66433-1-Ig (same clone as 66433-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

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  WB analysis using 66433-1-Ig (same clone as 66433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66433-1-Ig (same clone as 66433-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66433-1-Ig (same clone as 66433-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66433-1-Ig (same clone as 66433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66433-1-Ig (same clone as 66433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66433-1-Ig (same clone as 66433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66433-1-Ig (same clone as 66433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66433-1-PBS bindet in WB, IHC, Indirect ELISA Amphiregulin und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Amphiregulin fusion protein Ag8907
• Vollständiger Name: amphiregulin
• Berechnetes Molekulargewicht: 252 aa, 28 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 37 kDa
• GenBank-Zugangsnummer: BC009799
• Gene symbol: Amphiregulin/AREG
• Gene ID (NCBI): 374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).