Amylase Alpha Polyklonaler Antikörper

Amylase Alpha Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12540-1-PBS

Synonyme

AMY2B, Amylase, alpha, 1,4-alpha-D-glucan glucanohydrolase 2B, Alpha amylase, Alpha amylase 2B

---

• 

  WB analysis of mouse pancreas using 12540-1-AP (same clone as 12540-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12540-1-AP (Amylase, alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12540-1-AP (same clone as 12540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12540-1-AP (same clone as 12540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat pancreas using 12540-1-AP (same clone as 12540-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat pancreas tissue using Amylase Alpha antibody (12540-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12540-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Amylase Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Amylase Alpha fusion protein Ag3260
• Vollständiger Name: amylase, alpha 2B (pancreatic)
• Berechnetes Molekulargewicht: 511 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC011179
• Gene symbol: AMY2B
• Gene ID (NCBI): 280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. The full length 58 kDa protein has a signal peptide with 15 amino acids. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012). 12540-1-AP can recognize both AMY2A and AMY2B.