APP/Beta Amyloid Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

APP/Beta Amyloid Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(110)

Rezensionen (2)

Kat-Nr. : 25524-1-AP

Synonyme

amyloid precursor protein, APP, Alzheimer disease amyloid protein, Alzheimer disease amyloid A4 protein homolog, AD1

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  WB analysis using 25524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 25524-1-AP

  HAP1 (WT and APP KO) lysates prepared with RIPA buffer, 50μg protein loaded. 25524-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 25524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP/Beta Amyloid antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP/Beta Amyloid antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 25524-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP, C-Terminal antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HAP1 using 25524-1-AP

  HAP1 lysates prepared and IP of APP performed using 2.0 μg of 25524-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate.These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human gliomas using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of SH-SY5Y using 25524-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APP/Beta Amyloid antibody (25524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, C6-Zellen, HeLa-Zellen, Maushirngewebe, NCI-H1299-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 76 publications below
• IHC: See 21 publications below
• IF: See 28 publications below

Produktinformation

25524-1-AP bindet in WB, IHC, IF/ICC, ELISA APP/Beta Amyloid und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APP/Beta Amyloid fusion protein Ag22408
• Vollständiger Name: amyloid beta (A4) precursor protein
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC065529
• Gene symbol: APP
• Gene ID (NCBI): 351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This antibody can recognize several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP770, APP677, APP695, APP696, APP733, APP751, APP752, and APP639. APP can be cleaved into several chains, this antibody could recognize fragments C99, Amyloid-beta protein 42, Amyloid-beta protein 40, C83, P3(40), C80, Gamma-secretase C-terminal fragment 59, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 50, C31.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
mouse	IF	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard
human	WB	Small; Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease.; Authors - Qin Ouyang
rat	IHC	Biomater Res; Controlled release of Clenbuterol from a hydroxyapatite carrier for the treatment of Alzheimer's Disease; Authors - Yi-Wen Lin
human	WB	J Exp Clin Cancer Res; FOLR2+ macrophage depletion from intestinal metaplasia to early gastric cancer: single-cell sequencing insight into gastric cancer progression; Authors - Yuxin He
mouse	IHC	Redox Biol; Cognitive enhancement and neuroprotective effects of OABL, a sesquiterpene lactone in 5xFAD Alzheimer's disease mice model.; Authors - Jiang-Jiang Tang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jorge (Verified Customer) (03-28-2023)

Test to evaluate the efficacy of the antibody. Tested on SH-SY5Y APPswe cells differentiated with 10 uM retic acid for 5 days. DAPT treatment 10 uM for 24h. Cells lysed with RIPA buffer. Loading different protein concentrations (ug). Tricine SDS-PAGE 16% acrylamide gel. Transfered on PVDF membrane. Blocking 5% milk. Dilution 1:4000 in TBS-Tw 0.05%.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Cell line SHSY5Y APPswe

APP/Beta Amyloid Antibody Western Blot validation (1:4000 dilution) in Cell line SHSY5Y APPswe (Cat no:25524-1-AP)

FH

JIMMY (Verified Customer) (04-15-2019)

This antibody detects APP in the brain of 12 months C57BL/6 mice.

• Applications: Western Blot,