Angiogenin Rekombinanter Antikörper

Angiogenin Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242738D1

Kat-Nr. : 85635-1-RR

Synonyme

ANG, ANG 1, ANG I, ANG1, ANGI

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  WB analysis using 85635-1-RR

  Untreated and Brefeldin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 85635-1-RR (ANG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85635-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ANG antibody (85635-1-RR, Clone: 242738D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Brefeldin A treated HepG2 cells
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85635-1-RR bindet in WB, IF/ICC, ELISA Angiogenin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Angiogenin fusion protein Eg1930
• Vollständiger Name: angiogenin, ribonuclease, RNase A family, 5
• Berechnetes Molekulargewicht: 147 aa, 17 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC054880
• Gene symbol: Angiogenin
• Gene ID (NCBI): 283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.