Angiopoietin 1 Polyklonaler Antikörper

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• KD/KO Validated

Angiopoietin 1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

Konjugation

Unkonjugiert

Kat-Nr. : 23302-1-PBS

Synonyme

ANGPT1, AGP1, AGPT, ANG 1, ANG1

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  WB analysis using 23302-1-AP (same clone as 23302-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 23302-1-AP (same clone as 23302-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 23302-1-AP (same clone as 23302-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 23302-1-AP (same clone as 23302-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 23302-1-AP (same clone as 23302-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 23302-1-AP (same clone as 23302-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 23302-1-AP (same clone as 23302-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Angiopoietin 1 antibody (23302-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23302-1-PBS bindet in Angiopoietin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Angiopoietin 1 fusion protein Ag18829
• Vollständiger Name: angiopoietin 1
• Berechnetes Molekulargewicht: 498 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC152411
• Gene symbol: Angiopoietin 1
• Gene ID (NCBI): 284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.