Angiopoietin 1 Polyklonaler Antikörper

Angiopoietin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 27093-1-AP

Synonyme

ANGPT1, AGP1, AGPT, ANG 1, ANG1

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  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27093-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27093-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 27093-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Angiopoietin 1 antibody (27093-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, Mausherzgewebe, Rattenherzgewebe, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

27093-1-AP bindet in WB, IHC, IF/ICC, ELISA Angiopoietin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Angiopoietin 1 fusion protein Ag25786
• Vollständiger Name: angiopoietin 1
• Berechnetes Molekulargewicht: 498 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC152411
• Gene symbol: Angiopoietin 1
• Gene ID (NCBI): 284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publikationen

Species	Application	Title
human	WB	J Ethnopharmacol; Lycium barbarum polysaccharides attenuates high glucose-induced diabetic retinal angiogenesis by rescuing the expression of miR-15a-5p in RF/6A cells; Authors - Yafei Zhu
mouse	WB	Aging Cell; Phosphodiesterase inhibitor ameliorates senescent changes of renal interstitial pericytes in aging kidney; Authors - Hyung Duk Kim
rat	WB	Basic Res Cardiol; Investigating the cause of cardiovascular dysfunction in chronic kidney disease: capillary rarefaction and inflammation may contribute to detrimental cardiovascular outcomes; Authors - Siavash Beikoghli Kalkhoran
human	WB	Mater Today Bio; Doxorubicin-loaded PEGylated liposome modified with ANGPT2-specific peptide for integrative glioma-targeted imaging and therapy; Authors - Hongyan Li ; KD Validated
human	WB,IF	Adv Healthc Mater; Targeted Anti-Inflammatory Nanozymes with Pro-Angiogenic Activity for Myocardial Infarction Therapy; Authors - Wenling Yang