Angiopoietin-2 Polyklonaler Antikörper

Angiopoietin-2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31499-1-PBS

Synonyme

Angpt2, Agpt2, Ang 2, Ang2, angiopoietin 2

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  WB analysis using 31499-1-AP (same clone as 31499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 31499-1-AP (same clone as 31499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

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  IP experiment of rat lung using 31499-1-AP (same clone as 31499-1-PBS)

  IP result of anti-Angiopoietin-2 (IP:31499-1-AP, 4ug; Detection:31499-1-AP 1:3000) with rat lung tissue lysate 1200 ug. This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 31499-1-AP (same clone as 31499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 31499-1-AP (same clone as 31499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 31499-1-AP (same clone as 31499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 31499-1-AP (same clone as 31499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31499-1-PBS bindet in WB, IHC, IP, Indirect ELISA Angiopoietin-2 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Angiopoietin-2 fusion protein Eg0666
• Vollständiger Name: angiopoietin 2
• Berechnetes Molekulargewicht: 56KD
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: NM_007426.4
• Gene symbol: Angpt2
• Gene ID (NCBI): 11601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).