Angiopoietin-2 Rekombinanter Antikörper

Angiopoietin-2 Rekombinant Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240760D5

Kat-Nr. : 83816-1-RR

Synonyme

Angpt2, angiopoietin 2, Ang2, Ang 2, Agpt2

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  WB analysis using 83816-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 83816-1-RR

  IP result of anti-Angiopoietin-2 (IP:83816-1-RR, 4ug; Detection:83816-1-RR 1:1500) with mouse lung tissue lysate 1200 ug.

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  IHC staining of mouse kidney using 83816-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83816-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83816-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83816-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83816-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83816-1-RR against Mouse Angiopoietin-2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83816-1-RR bindet in WB, IHC, IP, ELISA Angiopoietin-2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: angiopoietin 2
• Berechnetes Molekulargewicht: 56KD
• Beobachtetes Molekulargewicht: 68kDa
• GenBank-Zugangsnummer: NM_007426.4
• Gene symbol: Angpt2
• Gene ID (NCBI): 11601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).