Angptl4 Polyklonaler Antikörper

Angptl4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 51109-1-AP

Synonyme

Hepatic fibrinogen/angiopoietin-related protein, ANGPTL4 N-terminal chain, ANGPTL4 C-terminal chain, Angiopoietin-related protein 4, Angiopoietin-like protein 4

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  WB analysis using 51109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51109-1-AP (Angptl4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausnierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IF: See 1 publications below

Produktinformation

51109-1-AP bindet in WB, IHC, IF, ELISA Angptl4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Angptl4 fusion protein Ag0613
• Vollständiger Name: angiopoietin-like 4
• Berechnetes Molekulargewicht: 405 aa, 45 kDa
• GenBank-Zugangsnummer: BC078944
• Gene symbol: Angptl4
• Gene ID (NCBI): 362850
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three functional domains: the signal peptide, the coiled-coil (N-terminal chain, CCD), and the fibrinogen-like (C-terminal chain, FLD) domains. ANGPTL4 is a glycosylated secreted protein (fANGPTL4, 45-65 kDa). fANGPTL is then rapidly post-translationally cleaved into an N-terminal coiled-coil domain (nANGPTL4, ~ 15 kDa) and a C-terminal fibrinogen-like domain (cANGPTL4, ~ 35 kDa)(PMID: 37300585 ).

Publikationen

Species	Application	Title
human,mouse	IF	Theranostics; Advancing the clinical assessment of glomerular podocyte pathology in kidney biopsies via super-resolution microscopy and angiopoietin-like 4 staining; Authors - Xiaojing Liu ; KO Validated