Annexin A3 Monoklonaler Antikörper

Annexin A3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1A11E4

Kat-Nr. : 66405-1-Ig

Synonyme

ANXA3, 1A11E4, 35 alpha calcimedin, 35-alpha calcimedin, Annexin 3

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  WB analysis using 66405-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 66405-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66405-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66405-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66405-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 66405-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 66405-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, Caco-2-Zellen, DU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Milzgewebe, L02-Zellen, SW 1990-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66405-1-Ig bindet in WB, IHC, IF, ELISA Annexin A3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Annexin A3 fusion protein Ag2381
• Vollständiger Name: annexin A3
• Berechnetes Molekulargewicht: 322 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC000871
• Gene symbol: Annexin A3
• Gene ID (NCBI): 306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Oncogene; Epstein-Barr virus causes vascular abnormalities in epithelial malignancies through upregulating ANXA3-HIF-1α-VEGF pathway; Authors - Yuanyuan Chen ; KO Validated