Annexin V Monoklonaler Antikörper

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• KD/KO Validated

Annexin V Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1E6A8

Kat-Nr. : CL594-66245

Synonyme

Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5

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  IF Staining of HeLa using CL594-66245

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Annexin V antibody (CL594-66245, Clone: 1E6A8 ) at dilution of 1:200.

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  IF Staining of HeLa using CL594-66245

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Annexin V antibody (CL594-66245, Clone: 1E6A8 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66245

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human Annexin V (CL594-66245, Clone:1E6A8) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66245 bindet in IF/ICC, FC (Intra) Annexin V und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Annexin V fusion protein Ag1538
• Vollständiger Name: annexin A5
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC001429
• Gene symbol: Annexin V
• Gene ID (NCBI): 308
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.