Annexin V Monoklonaler Antikörper

Annexin V Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1E6A8

Kat-Nr. : CL594-66245

Synonyme

Anchorin CII, Annexin 5, annexin A5, Annexin V, ANX5, ANXA5, Calphobindin I, CBP I, Endonexin II, ENX2, Lipocortin V, PAP I, PP4, Thromboplastin inhibitor, VAC alpha, Vascular anticoagulant alpha

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL594-66245

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Annexin V antibody (CL594-66245, Clone: 1E6A8 ) at dilution of 1:200.

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  IF Staining of HeLa using CL594-66245

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Annexin V antibody (CL594-66245, Clone: 1E6A8 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66245

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human Annexin V (CL594-66245, Clone:1E6A8) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66245 bindet in IF, FC (Intra) Annexin V und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Annexin V fusion protein Ag1538
• Vollständiger Name: annexin A5
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC001429
• Gene symbol: ANXA5
• Gene ID (NCBI): 308
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.